PID Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: PID Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-200-401-DN2
• Hersteller Artikelnummer: 200-401-DN2
• Alternativnummer: ROC-200-401-DN2
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC, WB
• Spezies Reaktivität: Human, Mouse, Rat
• Immunogen: Anti-PID antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid peptide near the C-terminus of human PID.
• Konjugation: Unconjugated
• Alternative Synonym: PID Antibody, PID, MTA1L1, PID, Metastasis-associated protein MTA2, Metastasis-associated 1-like 1, MTA1-L1 protein

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/mL by UV absorbance at 280 nm
• NCBI: 9219
• UniProt: O94776
• Puffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000, IHC: 2.5 µg/mL, IF Microscopy: 10 µg/mL, WB: 1 µg/mL
• Anwendungsbeschreibung: Anti-PID Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry, Immunohistochemistry, and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 75 kDa in West

Bilder

Immunocytochemistry of PID antibody. Cells: HeLa cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: PID antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: PID is nuclear. Staining: PID brown nuclear stain.

Immunofluorescence Microscopy of PID antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: PID antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: PID is nuclear. Staining: PID as red fluorescent signal.

Western Blot of PID antibody. Lane 1: HeLa whole cell lysate. Lane 2: HeLa whole cell lysate in the presence of blocking peptide. Load: 35 µg per lane. Primary antibody: PID antibody at 1 µg/mL for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 75 kDa, 73 kDa for PID. Other band(s): PID splice variants and isoforms.