Anti-Olig2 Antibody is tested for use in Western Blotting, Immunoprecipitation, and immunocytochemistry. Expect a band of approximately 27 kDa in size corresponding to Olig2 proteins in Western blots in the appropriate cell lysate or extracts.
Western Blot of Rabbit anti-Olig2 antibody. Lane 1: Neonatal Rat Brain Lysate. Lane 2: None. Load: 10 µg per lane. Primary antibody: Olig2 antibody at 1:3,000 for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 32 kDa for Olig2. Other band(s): Olig2 splice variants and isoforms.
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