Anti-BAG4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to amino acids near the N-terminus of human BAG4.
Konjugation:
Unconjugated
Alternative Synonym:
BAG-4, SODD, SODD Antibody, BAG family molecular chaperone regulator 4, Bcl-2-associated athanogene 4
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:5,000, IF Microscopy: 20 µg/mL, WB: .5 µg/mL
Anwendungsbeschreibung:
Anti-SODD Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 50 kDa in Western Blots of specific
Immunofluorescence Microscopy of BAG4 antibody. Tissue: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: BAG4 antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: BAG4 is cytoplasmic. Staining: BAG4 as red fluorescent signal.
Western blot of BAG4 antibody. Lane 1: HeLa whole cell lysate. Lane 2: THP-1 whole cell lysate. Lane 3: HeLa whole cell lysate in the presence of blocking peptide. Lane 4: THP-1 whole cell lysate in the presence of blocking peptide. Load: 35 µg per lane. Primary antibody: BAG4 antibody at 1:500 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 49.6 kDa, 64 kDa for BAG4. Other band(s): BAG4 splice variants and isoforms.
Immunocytochemistry of BAG4 antibody. Cells: HeLa cells. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: SODD antibody at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: SODD is cytoplasmic. Staining: BAG4 as precipitated brown signal with hematoxylin blue nuclear counterstain.
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