Histone H3 K27 methyl Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27 me3 site of human Histone H3.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
WB: 1:1000
Anwendungsbeschreibung:
Anti-Histone H3 K27 Methyl Antibody has been tested by dot blot and is suitable for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~15.4kDa corresponding to the appropriate cell
Dot Blot of Rabbit Histone H3 K27 me1-3 ac Antibody. Lane 1: Unmodified. Lane 2: me1. Lane 3: me2. Lane 4: me3. Lane 5: ac. Load: 1, 10, and 100 picomoles of peptide. Primary antibody: Histone H3 [1-3 methyl, Lys27, ac] antibody at 0.5µg/ml for 45 min at 4C. Secondary antibody: Dylight(TM)488 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C.
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