SARS-CoV-2 Nucleocapsid (N) Protein Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: SARS-CoV-2 Nucleocapsid (N) Protein Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-200-401-MS4
• Hersteller Artikelnummer: 200-401-MS4
• Alternativnummer: ROC-200-401-MS4
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Virus
• Immunogen: Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody was produced by repeated immunizations with purified recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells.
• Konjugation: Unconjugated
• Alternative Synonym: 2019-nCoV, COVID-19, N-protein antibody, SARS CoV2 antibody, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2, rabbit anti-SARS CoV 2 Nucleocapsid Protein Antibody, sars-cov-2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 5.0 mg/ml by UV absorbance at 280 nm
• NCBI: 43740575
• UniProt: P0DTC9
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: SARS
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: User Optimized, WB: 1:1000-1:2000
• Anwendungsbeschreibung: Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to SARS-CoV-2 Nucleocaps

Bilder

Western Blot Results of Rabbit Anti-SARS CoV-2 Nucleocapsid (N) Protein Antibody. Lane 1: Opal Prestained Molecular Weight (p/n MB-210-0500). Lane 2: SARS CoV-2 N protein (10ng). Primary Antibody: Anti-SARS CoV-2 N Protein at 1µg/mL overnight for 2 days at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP conjugated Antibody (p/n 611-1302) at 1:40,000 for 30 mins at RT. Block: BlockOut Buffer (p/n MB-073) for 1hr at RT. Predicted MW: ~47kDa, ~55kDa for glycosylated CoV-2-His-tag. Observed MW: ~63kDa. Other bands are due to overload of protein. Exposure: 1 sec.

Binding mode and kinetic analysis of Rabbit Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody (p/n 200-401-MS4). Using Octet R4, SARS-COV-2N was immobilized at 20 µg/mL on an AR2G biosensor and exposed to Rabbit Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody over a range of 2.0 nM - 16.7 µM. Each curve represents the acquired signal of its protein concentration. The interaction fits to a bivalent binding site kinetic model. The association constant (ka=3.8x10 3 M -1 s -1) and dissociation constant (kd=1.3x10 -4 s -1) values are depicted in the graph. The assay was performed as per the manufacturers protocol.

ELISA results of Rabbit Anti-SARS CoV-2 Nucleocapsid (N) Protein. Each well was coated in duplicate with 0.5 µg of SARS CoV-1 N [Red Line], SARS CoV-2 N [Green Line], MERS N [Blue Line]. The starting dilution of antibody was 2µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. SARS CoV-1 N IC50: 61ng/mL, SARS CoV-2 N IC50: 36ng/mL. Assay performed using Goat Anti-Rabbit IgG HRP conjugated (p/n 611-1302) and TMB substrate (p/n TMBE-1000).

Fitting of Kinetic Data to bivalent binding site model. Steady state signals are fitted to a bivalent binding site model. A 4-parameter non-linear regression curve was used for the integration of the data. The Rabbit Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody (p/n 200-401-MS4) presents a KD=35 µM (affinity constant) to Anti-SARS-CoV-2 Nucleocapsid (N) Protein.