Setdb2 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Setdb2 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-200-401-N99S
• Hersteller Artikelnummer: 200-401-N99S
• Alternativnummer: ROC-200-401-N99S
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human, Mouse
• Immunogen: Setdb2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length protein corresponding to mouse Setdb2.
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-Setdb2 Antibody, Histone-lysine N-methyltransferase SETDB2, SET domain bifurcated 2, Setdb 2 antibody, Gm293

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.98 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Mouse
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,500 - 1:10,000, WB: 1:10,000 - 1:100,000
• Anwendungsbeschreibung: Anti-Setdb2 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80.6 kDa in size corresponding to Setdb2 by western blotting in the appropriat

Bilder

Western Blot of Rabbit anti-Setdb2 antibody. Lane 1: 50ng recombinant mouse Setdb2. Lane 2: 50ng recombinant mouse Setdb2 + HEK293 50mcg. Lane 3: 5.0ng recombinant mouse Setdb2 + HEK293 50mcg. Lane 4: 0.5ng recombinant mouse Setdb2 + HEK293 50mcg. Lane 5: HEK293 50mcg. Lane 6: 50ng recombinant mouse Setdb2. Lane 7: 50ng recombinant mouse Setdb2 + HEK293 50mcg. Lane 8: 5.0ng recombinant mouse Setdb2 + HEK293 50mcg. Lane 9: 0.5ng recombinant mouse Setdb2 + HEK293 50mcg. Lane 10: HEK293 50mcg. Primary antibody: Setdb2 antibody at 1:100,000 (lanes 1-5) and 1:25,000 (lanes 6-10) for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 80.6 kDa for Setdb2. Other band(s): degradation products, proteolysis products, or nonspecific binding.