Histone H3 K4me1 Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-200-401-P02S
Artikelname: Histone H3 K4me1 Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-200-401-P02S
Hersteller Artikelnummer: 200-401-P02S
Alternativnummer: ROC-200-401-P02S
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: DOT, ELISA, WB
Spezies Reaktivität: Human
Immunogen: Histone H3 K4me1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic monomethylated peptide surrounding Lysine 4 of human Histone H3.2.
Konjugation: Unconjugated
Alternative Synonym: rabbit anti-Anti-Histone H3 Monomethyl Lys4 antibody, rabbit anti-Histone H3 K4 me1 antibody, H3 K4me1, H3.3B, H3 histone, family 3A, H3.3AH3F3H3F3B, histone H3.3, MGC87783, MGC87782
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
NCBI: 126961
UniProt: Q71DI3
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:3,000 - 1:10,000, ChIP: 2-5µg/million cells, IHC: 2 mg/ml - 5 µg/ml, IF Microscopy: 2 - 5 µg/ml, WB: 1:500 - 1:2,000
Anwendungsbeschreibung: Anti-Histone H3 [Monomethyl Lys4] antibody has been tested in ELISA, Dot blot, and Western Blot. Histone3 K4me1 is useful for Western Blot, Immunocytochemistry, Immunofluorescence, Chromatin Immunoprecipitation, and Dot Blot. Specific conditions for reac
Western Blot of Rabbit anti-Histone H3 K4Me1 antibody. Lane 1: Raji Whole Cell Lysate (p/n W09-001-368). Load: 35 µg per lane. Primary antibody: H3K4me1 antibody at 1:2000 for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:5,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~15kDa for Histone H3.