Dextranase (Penicillium Species) Antibody Biotin Conjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Dextranase (Penicillium Species) Antibody Biotin Conjugated, Rabbit, Polyclonal
• Artikelnummer: ROC-200-406-089S
• Hersteller Artikelnummer: 200-406-089S
• Alternativnummer: ROC-200-406-089S
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Algae
• Immunogen: Dextranase [Penicillium species] DEX
• Konjugation: Biotin
• Alternative Synonym: rabbit anti-Dextranase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Dextranase Antibody, dextranase, Alpha-1,6-glucan-6-glucanohydrolase, DEX

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 47720
• UniProt: P48845
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Penicillium
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:500 - 1:5,000
• Anwendungsbeschreibung: Anti-Dextranase Biotin Antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Dextranase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic

Bilder

ELISA Results of Rabbit Anti-Dextranase Antibody Biotin Conjugated tested against purified Dextranase protein. Each well was coated in duplicate with 1.0 µg of Dextranase. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer (p/n MB-076), Streptavidin-HRP conjugated (p/n S000-03), and TMB substrate (p/n TMBE-1000).

Western Blot of Anti-Dextranase Antibody Biotin Conjugated. Lane 1: Molecular Weight Marker. Lane 2: Dextranase. Primary Antibody: Rabbit Anti-Dextranase Biotin Conjugate at 1:1000 overnight at 2-8C. Secondary Antibody: Streptavidin-HRP (p/n S000-03) at 1:40,000 for 30 min at RT. Block: BlockOut Buffer (p/n MB-073). Expected MW: ~66kDa.