Anti-beta-2-Microglobulin Biotin antibody has been tested by dot blot and is suitable for western blotting to detect a single band of the expected apparent molecular weight and for ELISA. Researchers should determine optimal titers for applications that
Dot Blot results of Rabbit Anti-Beta 2 Microglobulin Biotin Conjugated. Antigen: Beta-2-Microglobulin. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Beta-2-Microglobulin Biotin 10µg/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRP (p/n S000-03) at 1:40,000 for 30min at RT. Imaging System ChemiDoc, Filter used: Chemi.
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