Maltose Binding Protein (MBP) Epitope Tag Antibody Biotin Conjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Maltose Binding Protein (MBP) Epitope Tag Antibody Biotin Conjugated, Rabbit, Polyclonal
• Artikelnummer: ROC-200-406-385S
• Hersteller Artikelnummer: 200-406-385S
• Alternativnummer: ROC-200-406-385S
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with the MBP epitope tag recombinant protein.
• Konjugation: Biotin
• Alternative Synonym: rabbit anti-MBP Epitope Tag Antibody biotin conjugation, rabbit anti-Maltose Binding Protein Antibody biotin conjugated

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000-1:50,000, WB: 1:1,000-1:5,000
• Anwendungsbeschreibung: Anti-MBP Biotin Conjugated Antibody is optimally suited for monitoring the expression of MBP tagged fusion proteins. As such, anti- MBP/MBP can be used to identify fusion proteins containing the MBP epitope. The antibody recognizes the MBP epitope tag fu

Bilder

Simplified diagram of MBP-fusion protein construct using pMal expression vector system.

Western Blot showing detection of Maltose Binding Protein (MBP) (0.05 µg) in Lane 2. MW markers indicated in Lane 1. Protein was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20C Anti-MBP (RABBIT) antibody (p/n 200-401-385) was used at 1:1000 overnight at 4C. Anti-Rabbit IgG (GOAT) IRDye800 conjugated antibody (p/n 611-131-002) secondary antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) for 30 min at 20C and imaged on the LiCor Odyssey imaging system. A band is present at the correct molecular weight, ~42 kDa, the other bands present are recombinant MBP breakdown.

Anti-MBP epitope tag polyclonal antibody detects MBP-tagged recombinant proteins by western blot. Polyclonal rabbit-anti-MBP epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the MBP epitope tag. The apparent molecular weight of this band is 42 kDa. A minor band at corresponding to multimers of this protein is also evident. A 4-20% gradient gel was used to separate the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] (code 611-132-122) for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.