Peroxidase Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Peroxidase Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-200-4138S
• Hersteller Artikelnummer: 200-4138S
• Alternativnummer: ROC-200-4138S
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Immunogen: Peroxidase [Horseradish]
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-Peroxidase Antibody, rabbit anti-HRP antibody, horseradish antibody, rabbit anti Peroxidase, rabbit anti HRP

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/ml by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:12,000 - 1:54,000, WB: 1:500 - 1:3000
• Anwendungsbeschreibung: Anti-Peroxidase antibody has been tested by western blotting. Specific conditions for reactivity should be optimized by the end user.

Bilder

Western Blot Results of Rabbit Anti-Peroxidase Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-20-0500). Lane 2: Peroxidase (HRP). Primary Antibody: Anti-HRP at 1:100in 0.75%Casein/TBST overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG mx10 DyLight(TM)649 (p/n 611-143-122) at 1:5000 in MB-070 Buffer for 30 mins at RT. Block: 0.75%Casein/TBST for 30 min at RT. Expected MW: ~44kDa.