Myeloperoxidase Antibody, Rabbit, Polyclonal

Artikelnummer: ROC-200-4181
Artikelname: Myeloperoxidase Antibody, Rabbit, Polyclonal
Artikelnummer: ROC-200-4181
Hersteller Artikelnummer: 200-4181
Alternativnummer: ROC-200-4181
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Human
Immunogen: Myeloperoxidase [Human Leukocytes]
Konjugation: Unconjugated
Alternative Synonym: rabbit anti-Myeloperoxidase Antibody, rabbit anti-MPO antibody
Klonalität: Polyclonal
Konzentration: 5.0 mg/mL by UV absorbance at 280 nm
NCBI: 4353
UniProt: P05164
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
Anwendungsbeschreibung: This antibody is tested by western blotting suitable for ELISA and immunoprecipitation. Although not tested, this antibody is likely functional in immunohistochemistry and other immunological methods. Anti-Human Myeloperoxidase may react with MPO from ot
Western Blot of Rabbit anti-Myeloperoxidase antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-364). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A549 Lysate (p/n W09-001-372). Lane 6: HL-60 Lysate (p/n W09-001-GL3). Lane 7: Raji Lysate (p/n W09-001-368). Lane 8: NIH/3T3 Lysate (p/n W10-000-358). Load: 35 µg per lane. Primary antibody: Myeloperoxidase antibody at 1:10,000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n 611-103-122) at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS (p/n MB-082) for 30 min at RT. Predicted/Observed size: 84kDa for Myeloperoxidase.