Anti-Lysozyme Peroxidase Conjugate Antibody has been tested by western blotting and ELISA and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below.
Western Blot of Rabbit anti-Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: ~1 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: MB-070 overnight at 4C. Predicted/Observed size: ~14-16kDa for Lysozyme. Other band(s): none.
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