0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Target-Kategorie:
Bovine
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:1,000 - 1:10,000, WB: 1:500 - 1:2,000
Anwendungsbeschreibung:
Anti-Alkaline Phosphatase Biotin Conjugated Antibody has been tested by ELISA and Western blot. This product is assayed against 1.0 ug of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-az
Western Blot of Rabbit anti-Alkaline Phosphatase antibody Biotin Conjugated. Lane 1: Alkaline Phosphatase (calf intestine). Load: 50 ng per lane. Primary antibody: Alkaline Phosphatase antibody Biotin conjugated at 1:2,000 for 60 min at RT. Secondary antibody: Peroxidase Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at RT. Predicted/Observed size: 57 kDa, 57 kDa for Alkaline Phosphatase.
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