0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Target-Kategorie:
Sweet Potato
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000
Anwendungsbeschreibung:
Anti-Beta Amylase Biotin Conjugated Antibody has been tested by dot blot and western blot. This product is assayed against 1.0 ug of b-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethyl
Dot Blot results of Rabbit Anti-Beta Amylase Biotin Conjugated. Antigen: Beta Amylase. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Beta Amylase Biotin 1µg/mL for 1hr at RT. Secondary Antibody: Goat anti-Rabbit HRP (p/n 611-103-122) at 1:70,000 for 30min at RT. Imaging System ChemiDoc, Filter used: Chemi.
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