Anti-Glutamate Dehydrogenase Antibody Biotin Conjugated, Rabbit, Polyclonal

Artikelnummer: ROC-200-4658-0100
Artikelname: Anti-Glutamate Dehydrogenase Antibody Biotin Conjugated, Rabbit, Polyclonal
Artikelnummer: ROC-200-4658-0100
Hersteller Artikelnummer: 200-4658-0100
Alternativnummer: ROC-200-4658-0100
Hersteller: Rockland Immunochemicals
Wirt: Rabbit
Kategorie: Antikörper
Applikation: DOT
Spezies Reaktivität: Bovine
Immunogen: Glutamate Dehydrogenase [Bovine Liver]
Konjugation: Biotin
Alternative Synonym: rabbit anti-Glutamate Dehydrogenase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Glutamate Dehydrogenase Antibody, Glutamate dehydrogenase 1 mitochondrial, GDH 1
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
NCBI: 281785
UniProt: P00366
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Bovine
Antibody Type: Primary Antibody
Application Verdünnung: IP: 1:100, WB: 1:500 - 1:2,000
Anwendungsbeschreibung: Anti-Glutamate Dehydrogenase Biotin Conjugated has been tested by dot blot and is suitable to be assayed against 1.0 ug of Glutamate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-e
Dot Blot results of Rabbit Anti-Glutamate Dehydrogenase Biotin Conjugated. Antigen: Glutamate Dehydrogenase. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Glutamate Dehydrogenase Biotin 1µg/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRP (S000-03) at 1:40,000 for 30min at RT. Imaging System ChemiDoc, Filter used: Chemi.