0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Bovine
Antibody Type:
Primary Antibody
Application Verdünnung:
IP: User Optimized, WB: User Optimized
Anwendungsbeschreibung:
Anti-Glutamate Dehydrogenase Biotin Conjugated has been tested by dot blot and is suitable to be assayed against 1.0 ug of Glutamate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-e
Dot Blot results of Rabbit Anti-Glutamate Dehydrogenase Biotin Conjugated. Antigen: Glutamate Dehydrogenase. Blot loaded at 3 fold dilution: 1. 100ng, 2. 33.3ng, 3. 11.1ng, 4. 3.70ng, 5. 1.23ng. Blocking: MB-070 Buffer for 30 minutes at RT. Primary Antibody: Rabbit Anti-Glutamate Dehydrogenase Biotin 1µg/mL for 1hr at RT. Secondary Antibody: Streptavidin-HRP (S000-03) at 1:40,000 for 30min at RT. Imaging System ChemiDoc, Filter used: Chemi.
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