Lysozyme Antibody Biotin Conjugated, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Lysozyme Antibody Biotin Conjugated, Rabbit, Polyclonal
• Artikelnummer: ROC-200-4672S
• Hersteller Artikelnummer: 200-4672S
• Alternativnummer: ROC-200-4672S
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Gallus
• Immunogen: Anti-Lysozyme Antibody was produced by repeated immunizations with hen egg white Lysozyme protein.
• Konjugation: Biotin
• Alternative Synonym: rabbit anti-Lysozyme Antibody biotin Conjugation, biotin Conjugated rabbit anti-Lysozyme Antibody, 1 4 beta n acetylmuramidase c antibody, EC 3.2.1.17 antibody, LYZ antibody, Lysozyme C antibody, Lysozyme C precursor antibody, LZM antibody, Hen Egg White

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 396218
• UniProt: P00698
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Chicken
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,000, IHC: 1:100 - 1:500, IP: 1:100, WB: 1:200 - 1:1,000
• Anwendungsbeschreibung: Anti-Lysozyme Biotin Conjugated Antibody has been tested by ELISA and western blot and is suitable for IP. Researchers should determine optimal titers for applications that are not stated below.

Bilder

Western Blot of Rabbit anti-Lysozyme antibody. (Left Blot-grey) Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Rockland Biotin Conjugated Rabbit anti Lysozyme antibody (200-4672 lot 20676 1:5K in MB-0070, ON 4 C). Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: ~1 ug per lane. Primary antibody: Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Streptavidin at 1:1,000 with Block: MB-070 overnight at 4C. (Right Blot-red) Blot shown on right shows detection of 0.5 ug reduced (R) and nonreduced (NR) Lysozyme using Rockland p/n 200-401-072 lot 26517 (1:1000 in MB-070) with Rockland Dylight 549 conjugated secondary antibody (605-742-125 lot 21096 1:10K 1.5 hr RT in MB-070). Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Predicted/Observed size: ~14-16 kDa for Lysozyme. Other band(s): none.