Anti-CDC42 antibody was prepared from chicken egg fractions produced by repeated immunizations with a 17 amino acid synthetic peptide near the N-terminus of human CDC42.
Konjugation:
Unconjugated
Alternative Synonym:
CDC42 Antibody, G25K, CDC42Hs, Cell division control protein 42 homolog, G25K GTP-binding protein
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:5000 - 1:10000, WB: 0.5-1 µg/mL
Anwendungsbeschreibung:
Anti-CDC42 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 26 kDa in Western Blots of specific cell lysates and tissues.
Western Blot of CDC42 antibody. Lane 1: Human brain tissue lysate with CDC42 antibody at 0.5 µg/mL. Lane 2: Human brain tissue lysate with CDC42 antibody at 1 µg/mL. Load: 35 µg per lane. Secondary antibody: Peroxidase chicken secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 26 kDa, 24 kDa for CDC42. Other band(s): none.
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