This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 79 amino acids of mature cat IL-8 protein.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Target-Kategorie:
Cat
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:10,000 - 1:50,000, WB: 1:500-1:2,000
Anwendungsbeschreibung:
This protein A purified CXCL8 antibody has been tested by Western blotting and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 8.9 kDa in size corresponding to cat IL-8 by western
Western blot using Rocklands protein-A purified anti-Cat IL-8 antibody shows detection of recombinant Cat IL-8 at 8.9 kDa, raised in yeast. Primary antibody was diluted to 1 µg/ml. 3% BSA from Rocklands BSA-30 (Bovine Serum Albumin Solution) was used for blocking. Secondary antibody 611-131-122 (Goat anti-Rabbit IgG IRDye 800) was used at 1:20,000.
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