This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 134 amino acids of mature cat IL-2.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Cat
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:20,000, WB: 1:500-1:2,000
Anwendungsbeschreibung:
This protein A purified IL-2 antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15.4 kDa in size corresponding to feline IL-2 by western blotting in
Western blot using Rocklands protein-A purified anti-Cat IL-2 antibody shows detection of recombinant Cat IL-2 at 15.4 kDa, raised in yeast. Primary antibody was diluted to 1 µg/ml. 3% BSA from Rocklands p/n BSA-30 (Bovine Serum Albumin Solution) was used for blocking. Secondary antibody p/n 611-131-122 (Goat anti-Rabbit IgG IRDye 800) was used at 1:20,000.
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