Progesterone Receptor phospho S190 Antibody, IgG1, Clone: [1154], Mouse, Monoclonal

Artikelnummer: ROC-209-301-E16
Artikelname: Progesterone Receptor phospho S190 Antibody, IgG1, Clone: [1154], Mouse, Monoclonal
Artikelnummer: ROC-209-301-E16
Hersteller Artikelnummer: 209-301-E16
Alternativnummer: ROC-209-301-E16
Hersteller: Rockland Immunochemicals
Wirt: Mouse
Kategorie: Antikörper
Applikation: IHC, WB
Spezies Reaktivität: Human
Immunogen: Anti-Progesterone Receptor pS190 Monoclonal Antibody was produced in mouse by repeated immunizations with Progesterone Receptor- Synthetic peptide corresponding to amino acid residues surrounding Ser190 conjugated to KLH.
Konjugation: Unconjugated
Alternative Synonym: mouse anti-Progesterone Receptor phospho S190 Antibody, mouse anti-Progesterone Receptor pS190 Antibody, PR, Nuclear receptor subfamily 3 group C member 3
Klonalität: Monoclonal
Klon-Bezeichnung: [1154]
Isotyp: IgG1
NCBI: 5241
UniProt: P06401
Puffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
Formulierung: Liquid
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: IHC: 1:1000, WB: 1:1000
Anwendungsbeschreibung: Anti-Progesterone Receptor pS190 (Mouse) antibody is tested for use in Western Blot and IHC. Expect a band of approximately 90 kDa in size for PR-A isoform and approximately 120 kDa in size for the PR-B isoform phosphorylated at Ser190 in Western Blottin
Western Blot of Mouse anti-Progesterone Receptor pS190 antibody. Lane 1: whole cell T47D lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM). Lane 2: none. Load: 10 µg per lane. Primary antibody: Progesterone Receptor pS190 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 90 kDa for Progesterone Receptor pS190. Other band(s): Progesterone Receptor pS190 splice variants and isoforms.