Anti-Progesterone Receptor pS294 (Mouse) Monoclonal Antibody was produced in mouse by repeated immunizations with Progesterone Receptor pS294- Synthetic peptide corresponding to amino acid residues surrounding Ser294 of the human progesterone receptor protein.
Konjugation:
Unconjugated
Alternative Synonym:
mouse anti-Progesterone Receptor phospho S294 Antibody, mouse anti-Progesterone Receptor pS294 antibody, PR, Nuclear receptor subfamily 3 group C member 3
Anti-Progesterone Receptor pS294 (Mouse) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should but optimized by the end user. Expect a band of approximately 90 kDa in size for PR-A isoform and the approximately
Western Blot of Mouse anti-Progesterone Receptor pS294 antibody. Lane 1: whole cell T47D lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM). Lane 2: none. Load: 10 µg per lane. Primary antibody: Progesterone Receptor pS294 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 90 kDa for Progesterone Receptor pS294. Other band(s): none.
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