IL-1 Alpha Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: IL-1 Alpha Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-209-401-302S
• Hersteller Artikelnummer: 209-401-302S
• Alternativnummer: ROC-209-401-302S
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Human
• Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli. The MW of the recombinant IL-1a was 17,000. This is the cleavage site generated by the IL-1b converting enzyme (ICE, capase-1).
• Konjugation: Unconjugated
• Alternative Synonym: Interleukin 1 alpha precursor antibody, rabbit anti-Interleukin1 alpha antibody, rabbit anti-IL-1 Alpha Antibody, Pre-interleukin 1 alpha antibody

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1 mg/ml by UV absorbance at 280 nm
• NCBI: 3552
• UniProt: P01583
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:1,000 - 1:5,000, IHC: 1:100 to 1:200, WB: 1:500 - 1:2,000
• Anwendungsbeschreibung: This IgG fraction antibody of anti-Human IL-1a has been tested for use in immunoblotting. This antibody is suitable for use in ELISA, neutralizations, immunohistochemistry, and flow cytometry. It recognizes the 17,000 MW mature IL-1a. Reactivity in other

Bilder

Western Blot of Rabbit Anti-Human IL-1 alpha Antibody. Lane 1: Opal Prestained Molecular Weight Ladder (p/n MB-210-0500). Lane 2: IL-1 alpha [50ng]. Lane 3: MEF WC lysate [10ng] (p/n W10-001-371). Lane 4: MEF LPS stimulated [10ng]. Blocking: BlockOut Buffer (p/n MB-073) for 30min at RT. Primary Antibody: Anti-IL-1a at 1µg/mL overnight at 4C. Secondary Antibody: Goat anti-Rabbit HRP (p/n 611-103-122) at 1:70, 000 for 30min at RT.