This antibody was prepared by repeated immunizations with recombinant human IL-1ß produced in E.coli. The MW of the recombinant 153 aa IL-1ß was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1ß converting enzyme (ICE, capase-1).
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000
Anwendungsbeschreibung:
Anti-Human IL-1ß has been tested in western blot. This antibody is suitable for use in neutralizations, ELISA, radioimmunoassays, flow cytometry, immunohistochemistry, and immunoprecipitation. It recognizes the 17,000 MW mature IL-1ß. For immunoblots, ty
Western blot showing detection of recombinant human IL-1ß by Rocklands anti-IL-1ß antibody. Recombinant human IL-1ß was loaded on to an SDS-PAGE gel at 0.1 µg and after separation, transferred to nitrocellulose. The expected band is approximately 18 kDa in size. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4C. After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
