IL-4 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: IL-4 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-209-401-B93
• Hersteller Artikelnummer: 209-401-B93
• Alternativnummer: ROC-209-401-B93
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Human
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-4 protein.
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-IL-4 antibody, rabbit anti-interleukin-4 antibody, BCGF-1, BCGF1, Binetrakin, BSF 1, BSF1, HCGF, IA Inducing Factor, IGG1 induction factor, IL 4, Il4e12, Interleukin 4, Interleukin 4, isoform 1, Interleukin4, Lymphocyte stimulatory factor 1, MCGF2, MFF, Pitrakinra, TCGF2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 3565
• UniProt: P05112
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000, WB: 1:1,000
• Anwendungsbeschreibung: This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band app

Bilder

Rocklands anti-Human IL-4 antibody shows detection of a band ~15 kDa in size corresponding to recombinant human IL-4. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax(TM) chemiluminescent substrate. Image was captured using VersaDoc(TM) MP 4000 imaging system (Bio-Rad).