Anti-IL-22 antibody was prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human IL-22 protein from E. coli.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:20,000 - 1:50,000, WB: 1:1000-1:2000
Anwendungsbeschreibung:
IL-22 purified antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 20 kDa in size corresponding to the human IL-22 protein by western blo
Western Blot of Rabbit anti-IL-22 antibody. Lane 1: Human IL-22 Recombinant Protein. Load: 50 ng per lane. Primary antibody: IL-22 antibody at 1:1,000 for overnight at 4C. Secondary antibody: DyLight(TM) 649 rabbit secondary antibody (611-143-002) at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 14 kDa, 14 kDa for Human IL-22. Other band(s): None.
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