IL-33 Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal

Produktübersicht

• Artikelname: IL-33 Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal
• Artikelnummer: ROC-209-403-B50
• Hersteller Artikelnummer: 209-403-B50
• Alternativnummer: ROC-209-403-B50
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: WB
• Spezies Reaktivität: Human
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.
• Konjugation: HRP
• Alternative Synonym: rabbit anti-Interleukin-33 peroxidase conjugated antibody, rabbit anti-IL-33 peroxidase conjugated antibody, Interleukin-1 family member 11, IL-1F11, nuclear factor from high endothelial venules and NF-HEV

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 90865
• UniProt: O95760
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: This purified antibody has been tested in western blotting and suitable for ELIAA. By western blot a band approximately 18 kDa in size corresponding to mature human IL-32alpha protein is expected in the appropriate cell lysate or extract. Specific conditions

Bilder

Western blot using Rocklands HRP conjugated anti-Human IL-33 antibody shows detection of a band ~18 kDa in size corresponding to recombinant human IL-33. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5,000 dilution for 30 min at room temperature. Detection occurred using TMB substrate.