IL-7 Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal

Produktübersicht

• Artikelname: IL-7 Antibody Peroxidase Conjugated, HRP, Rabbit, Polyclonal
• Artikelnummer: ROC-209-403-B94
• Hersteller Artikelnummer: 209-403-B94
• Alternativnummer: ROC-209-403-B94
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: DOT, WB
• Spezies Reaktivität: Human
• Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-7 protein.
• Konjugation: HRP
• Alternative Synonym: rabbit anti-IL-7 peroxidase conjugated antibody, rabbit anti-interleukin-7 antibody, IL7, IL-7 Interleukin 7, Interleukin-7

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• NCBI: 3574
• UniProt: P13232
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:5,000
• Anwendungsbeschreibung: This purified antibody has been tested in western blotting and ELISA. By western blot a band approximately 17.4 kDa in size corresponding to human IL-7 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity shou

Bilder

Rocklands anti-Human IL-7 antibody shows detection of a band ~17 kDa in size corresponding to recombinant human IL-7. The identity of the faint higher molecular weight band may represent a homodimer. Molecular weight markers are also shown (left). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (p/n 611-103-122) secondary antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax(TM) chemiluminescent substrate. Image was captured using VersaDoc(TM) MP 4000 imaging system (Bio-Rad).