This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to mature human NAG-1 protein. Cross reactivity to MBP was removed via cross-adsorption chromatography.
This Protein-A purified, MBP-cross-adsorbed NAG-1 Biotin conjugated antibody has ben tested by Western blot of human and mouse NAG-1 protein and is suitable for ELISA. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibo
Western Blot of Rabbit Anti-NAG-1 Biotin Conjugated Antibody. Lane 1: Rec. Human NAG1 (yeast expressed) reduced (0.1µg). Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500) (5µL). Lane 3: Rec. Human NAG1 (yeast expressed) non-reduced (0.1µg). Primary Antibody: Anti-NAG1 Biotin Conjugate at 1µg/mL overnight at 2-8C. Secondary Antibody: Streptavidin-HRP (p/n S000-03) at 1:40,000 for 30 min at RT. Blocking buffer: (p/n MB-070). Expect/Observed: ~14kDa.
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