Anti-Human IL-3 (RABBIT) Antibody Biotin Conjugated was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-3 protein.
This purified antibody has been tested in western blotting and suitable in ELISA. By western blot a band approximately 15 kDa in size corresponding to human IL-3 protein is expected in the appropriate cell lysate or extract. Specific conditions for react
Western Blot of Rabbit anti-Human IL-3 Biotin Conjugated Antibody. Lane 1: Human IL-3. Lane 2: None. Load: 50 ng per lane. Primary antibody: Human IL-3 primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 15 kDa, 15 kDa for Human IL-3. Other band(s): None.
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