This purified antibody has been tested in western blotting and suitable in ELISA. By western blot a band approximately 15 kDa in size corresponding to human IL-2 protein is expected in the appropriate cell lysate or extract. Specific conditions for react
Western Blot of Rabbit anti-Human IL-2 Biotin Conjugated Antibody. Lane 1: Human IL-2. Lane 2: None. Load: 50 ng per lane. Primary antibody: Biotin Human IL-2 primary antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 16 kDa, 16 kDa for Human IL-2. Other band(s): None.
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