Anti-EBI-3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Target-Kategorie:
Mouse
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:10,000, IP: 1µg/mL, WB: 1:1000
Anwendungsbeschreibung:
This purified antibody has been tested in Neutralization and suitable for IP. Specific conditions for reactivity should be optimized by the end user.
Neutralization: Human CD4+CD25- (Tconv) were purified by FACS from cord blood, activated with anti-CD3/CD28-coated beads and 10IU IL-2 in the presence of human IL-35 and a titration of an isotype control or 210-301-C56 antibody. Percent neutralization was determined by calculating the percent decrease in counts per minute of activated Tconv plus (0%) or minus (100%) human IL-35 in the presence and absence of Ab.
Neutralization: Murine CD4+CD25- (Tconv) were purified by FACS, activated with anti-CD3/CD28-coated beads in the presence of a titration of mouse IL-35 and 10µg/mL of 210-301-C56 antibody. Percent suppression was determined by calculating the percent decrease in counts per minute of activated Tconv plus or minus murine IL-35.
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