Anti-Synaptojanin 1 Monoclonal Antibody was produced in mouse by repeated immunizations with recombinant protein from the C-terminal region of rat synaptojanin 1.
Anti-Synaptojanin 1 antibody is tested for use in Western Blotting and IHC. Expect a band of approximately 145 kDa in size corresponding to synaptojanin I proteins of rat brain extracts. Researchers should determine optimal titers for applications that a
Western Blot of Mouse anti-Synaptojanin 1 antibody. Lane 1: rat hippocampal lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: Synaptojanin 1 antibody at 1:1,000 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 145 kDa for Synaptojanin 1. Other band(s): none.
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