0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Target-Kategorie:
Rat
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
Anwendungsbeschreibung:
Anti-Rat serum Peroxidase Conjugated antibody has been tested by Dot blot and ELISA and is suitable for western blotting, IP and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
ELISA Results of Rabbit Anti-Rat Serum Antibody Peroxidase Conjugated. Each well was coated in duplicate with 1.0 µg of Rat IgG (p/n 012-0102) [Red Line]. The working dilution is 20,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).
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