This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast, corresponding to 154 amino acids of mature swine TNFalpha.
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Target-Kategorie:
Swine
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:2,000 - 1:2,500, IHC: User Optimized, WB: 1:1000
Anwendungsbeschreibung:
This protein A purified TNFalpha antibody has been tested by western blot and is suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.9 kDa in size corresponding to swine TNFalpha by western b
Western blot using Rocklands protein-A purified anti-swine TNFa antibody shows detection of recombinant swine TNFa at 16.9 kDa, raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with 1 µg/mL primary antibody overnight at 4C. After washing, membrane was probed with IRDye800(TM) Conjugated Goat Anti-Rabbit IgG (p/n 611-132-122) for 45 min at room temperature.
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