Apolipoprotein E antibodies have been tested by western blot and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Western Blot of goat Anti Apolipoprotein E Antibody. Lane 1: rat glial whole cell lysate. Load: 40 µg per lane. Primary antibody: Apolipoprotein E antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP goat secondary antibody at 1:200 for 45 min at RT. Block: 3%BLOTTO overnight at 4C. Predicted/Observed size: 36 kDa, 35-36 kDa for APO-E. MW markers arrows at 25kDa and 37kDa. Other band(s): ~55kDa.
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