APOLIPOPROTEIN E Antibody, Goat, Polyclonal

Artikelnummer: ROC-600-101-197
Artikelname: APOLIPOPROTEIN E Antibody, Goat, Polyclonal
Artikelnummer: ROC-600-101-197
Hersteller Artikelnummer: 600-101-197
Alternativnummer: ROC-600-101-197
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: WB
Spezies Reaktivität: Human, Rat
Immunogen: Apolipoprotein Type E was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Konjugation: Unconjugated
Alternative Synonym: goat anti-Apolipoprotein E3 antibody, Alzheimer disease 2 antibody, Apo-E antibody, Apo E antibody, ApoE antibody, APOEA antibody, Apolipoprotein-E antibody, Apoprotein antibody
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
NCBI: 348
UniProt: P02649
Puffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Formulierung: Liquid (sterile filtered)
Target-Kategorie: Human
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:4,000 - 1:8,000, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:500 - 1:2,000
Anwendungsbeschreibung: Apolipoprotein E antibodies have been tested by western blot and used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Western Blot of goat Anti Apolipoprotein E Antibody. Lane 1: rat glial whole cell lysate. Load: 40 µg per lane. Primary antibody: Apolipoprotein E antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP goat secondary antibody at 1:200 for 45 min at RT. Block: 3%BLOTTO overnight at 4C. Predicted/Observed size: 36 kDa, 35-36 kDa for APO-E. MW markers arrows at 25kDa and 37kDa. Other band(s): ~55kDa.