This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 675-700 of Human AP3D1.
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to AP3D1 by western blotting in the ap
Western blot using Rocklands Affinity Purified anti-AP3D1 antibody shows detection of a 130-kDa band corresponding to Human AP3D1 in a HeLa whole cell lysate (p/n W09-000-364). The lower molecular weight band most likely represents non-specific binding. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP3D1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG (p/n 605-4302) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 30-sec exposure time.
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