Anti-Avian Influenza Hemagglutinin 3 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to 14 amino acids near the internal region of the Hemagglutinin protein Efforts were made to use relatively conserved regions of the viral sequence as the antigen.
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
H5N1 virus
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:10,000, WB: 1 µg/mL
Anwendungsbeschreibung:
Anti-Avian Influenza Hemagglutinin 3 Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 64 kDa in Western Blots of specific cell lysates a
Western Blot of Avian Influenza Hemagglutinin 3 antibody. Lane 1: 1 ng of recombinant HA1 with Avian Influenza Hemagglutinin 3 antibody. Lane 2: 5 ng of recombinant HA1 with Avian Influenza Hemagglutinin 3 antibody. Load: 35 µg per lane. Primary antibody: Avian Influenza Hemagglutinin 3 antibody at 1 µg/mL for overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 64 kDa, 45 kDa for Avian Influenza Hemagglutinin 3. Other band(s): Avian Influenza Hemagglutinin 3 splice variants and isoforms.
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