GST Antibody Alkaline Phosphatase Conjugated, ALP, Goat, Polyclonal

Artikelnummer: ROC-600-105-200
Artikelname: GST Antibody Alkaline Phosphatase Conjugated, ALP, Goat, Polyclonal
Artikelnummer: ROC-600-105-200
Hersteller Artikelnummer: 600-105-200
Alternativnummer: ROC-600-105-200
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: All
Immunogen: Glutathione-S-Transferase [Schistosoma japonicum]
Konjugation: ALP
Alternative Synonym: goat anti-GST antibody alkaline phosphatase conjugation, alk phos conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody, ALP, Alk Phos
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
Formulierung: Liquid (sterile filtered)
Antibody Type: Primary Antibody
Application Verdünnung: ELISA: 1:1,000 - 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
Anwendungsbeschreibung: Anti-GST Antibody Alkaline Phosphatase conjugated has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry as well as other antibody based assays using alkaline phosphatase conjugates requiring lot-to-lot
Western Blot of Goat anti GST antibody. Lane 1: molecular weight. Lane 2: 1 ug GST reduced. Lane 3: 0.25 ug GST reduced. Lane 4: 1 ug GST non-reduced. Lane 5: 0.25 ug GST non-reduced. Lane 6: molecular weight. Primary antibody: Goat anti GST antibody at 1:5000 for overnight at 4C. Secondary antibody: Dylight 649 conjugated Donkey anti Goat secondary antibody at 1:10,000 for 1.5 hours at RT. Lane 7: 1 ug GST reduced. Lane 8: 1 ug GST non-reduced. Lane 9: molecular weight. Primary antibody: Goat anti GST antibody at 1:1000 for overnight at 4C. Secondary antibody: Dylight 549 conjugated Donkey anti Goat secondary antibody at 1:10,000 for 1.5 hours at RT. Block: MB-070 overnight at 4C.