This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed gracilaria.
Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate into compo
Immunohistochemistry of Anti-Phycoerythrin Antibody Alkaline Phosphatase Conjugated.Less albumin extravasation in HDM-treatedPDE3-/-mice compared with WT mice. (D-I) and *20 magnification (J-L). Albumin positivity can be seen throughout the entirety of the lung tissue, but is mainly sequestered inside the large blood vessels, perivascular as well as peribronchial. Mucus was also stained for albumin. PBS-treated mice (A-C) were indistinguishable from each other concerning albumin content. WT HDM-treated mice (GandJ) showed albumin positivity surrounding the bronchioles when compared withPDE3-/-mice (H,I,K, andL). Original magnification, *100. Zooming in on (*400) the peribronchial region (highlighted with white rectangle), the smaller vasculature surrounding the bronchiole is albumin-positive in WT mice (J) (highlighted with white arrows) when compared with thePDE3-/-mice (KandL). Kruskal-Wallis test for multiple comparisons was used followed by Mann-WhitneyUtest. Data represent 2 separate experiments (n= 3 for all PBS groups,n= 7 for WT HDM,n= 5 for bothPDE3A-/-andPDE3B-/-HDM groups) and are shown as the mean SEM. *P< 0.05. Figure 3. PMID: 29367458.
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