Anti-Fluorescein Antibody Biotin Conjugated has been tested by ELISA, dot blot and western blot and is suitable for immunoblotting, ELISA, in situ hybridization, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptav
Western Blot of Biotin Conjugated Goat Anti-Fluorescein Antibody. Lane 1: BSA conjugated Fluorescein. Lane 2: None. Load: 50 ng per lane. Primary antibody: Fluorescein antibody at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase conjugated Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 70 kDa, 70 kDa for BSA-FITC. Other band(s): None.
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