Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Konjugation:
Texas Red
Alternative Synonym:
mouse anti-GFP antibody Texas Red(TM) conjugation, Texas Red(TM) conjugated mouse anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Lyophilized
Antibody Type:
Primary Antibody
Application Verdünnung:
FLISA: >1:20,000, IF Microscopy: 1:500 - 1:2,500, WB: >1:10,000
Anwendungsbeschreibung:
Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody has been tested by dot blot. It can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated mo
Dot Blot results of Mouse Anti-GFP Antibody Texas Red(TM) Conjugated. Dots are GFP at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 60 min at RT. Primary Antibody: none. Secondary Antibody: Mouse Anti-GFP Antibody Texas Red(TM) at 1µg/mL for 1hr at RT. Imaged with BioRad ChemiDoc, DL549 filter.
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