Anti-Human ALPHA-1-ANTI-TRYPSIN Antibody has been tested by ELISA, and western blot. This product is assayed against 1.0 µg of Human Alpha-1-anti-Trypsin in a standard ELISA using Peroxidase conjugated Affinity Purified Donkey anti-Rabbit IgG and TMB as
Western Blot of Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent Western blot. Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin (green), and Mouse-a-GST (blue) were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 µg per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4C. Secondary antibody: Donkey anti-Rabbit IgG DyLight 549 (p/n 611-742-127), Donkey anti-Mouse IgG DyLight 488 (p/n 610-741-002), and Donkey anti-Goat IgG DyLight 649 (p/n 605-743-002) secondary antibody at 1:10,000 for 30 min at RT. Block: (p/n MB-070) at 30 min RT.
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