Human Haptoglobin Antibody is tested by ELISA and is suitable for western blotting, ELISA and IHC. Researchers should determine optimal titers for applications that are not stated below.
ELISA results of purified Rabbit anti-Human Haptoglobin Antibody tested against immunizing antigen. Each well was coated in duplicate with 1.0 µg of antigen. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin (p/n MB-066) as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n 611-103-122) and TMB substrate (p/n TMBE-1000).
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