This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 80-115 of Human FANCC.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-FANCC antibody, FANC-C, FANC C, FAC antibody, FACC antibody, Fanconi anemia group C protein, Fanconi anemia complementation group C antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:20,000 - 1:80,000, WB: 1:1,000 - 1:3,000
Anwendungsbeschreibung:
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 63 kDa in size corresponding to FANCC by western blotting in the app
Western blot using Rocklands affinity purified anti-FANCC antibody shows detection of a band at ~63 kDa (arrowhead) corresponding to FANCC present in a HeLa whole cell lysate (p/n W09-000-364). The identity of the lower molecular weight band is unknown. Approximately 35µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4C with the primary antibody diluted to 1:1,500 in PBS supplemented with 1% normal goat serum and 0.1% BLOTTO (p/n B501-0500). The membrane was washed and reacted with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] (p/n 611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers (indicated at left). IRDye(TM)800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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