This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 63-76 of Human Elg 1.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-ELG-1 antibody, ELG1, ELG 1, FRAG1, ATP (GTP)-binding protein, ATPase Family AAA Domain-Containing Protein 5, Chromosome fragility associated gene 1 protein, ATAD5, C17orf41
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:3,000 - 1:15,000, WB: 1:500 - 1:2,000
Anwendungsbeschreibung:
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to Elg1 by western blotting in the approp
Western blot using Rocklands Affinity Purified anti-Elg1 antibody shows detection of a band ~120 kDa corresponding to human Elg1 in various cell lysates. Lane 1: HeLa nuclear extract (p/n W09-001-367), Lane 2: HeLa (p/n W09-000-364), Lane 3: A431 (p/n W09-000-361), Jurkat (p/n W09-001-370) and Lane 5: HEK293 (p/n W09-000-365) whole cell lysates. Load: ~5µg per lane. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2 m exposure time. The expected molecular weight of Elg1 is 120kDa according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kDa. The 50kDa bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 kDa and 50 kDa bands are not observed when antibody is pre-incubated with peptide (data not shown).
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