DIA-2 Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: DIA-2 Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-600-401-851
• Hersteller Artikelnummer: 600-401-851
• Alternativnummer: ROC-600-401-851
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Human
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues at the C-Terminus of Human DIA-2.
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-DIA-2 Antibody, DIA 2, DIA drome, DIA2, DIAPH2, Diaphanous 2, Diaphanous related formin 2, Diaphorase 2, DRF2, POF, POF2

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.4 mg/mL by UV absorbance at 280 nm
• NCBI: 1730
• UniProt: O60879
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:12,000 - 1:60,000, IP: 1:100, WB: 1:1,000 - 1:6,000
• Anwendungsbeschreibung: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 125 kDa in size corresponding to DIA-2 by western blotting in the ap

Bilder

Western blot using Rocklands Affinity Purified anti-DIA-2 antibody shows detection of a 132-kDa band corresponding to DIA-2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.