This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal region near aa 150-183 of Human UBC12 protein.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-UBC12 antibody, UBC-12, UBC 12, NEDD-8, NEDD 8, NEDD8 carrier protein antibody, NEDD8 conjugating enzyme Ubc12 antibody, NEDD8 protein ligase antibody, UBE2M antibody, Ubiquitin conjugating enzyme E2 M antibody
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,000
Anwendungsbeschreibung:
UBC12 Antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to UBC12 by western blotting in the appropriate cell lys
Panel A shows the portion of the protein unique to UBC12 and common to all members of the E2 ubiquitin-conjugating enzyme family. Panel B shows UBC12s involvement in UBL and NEDD8 conjugation cascades. See Huang et al for more details.
Western blot analysis is shown using Rocklands Affinity Purified anti-UBC12 antibody to detect Human UBC12 in various preparations. This western blot shows reactivity with purified human UBC12 protein (lane 1), NIH-3T3 cells over expressing UBC12 by infection (lane 2) and endogenous UBC12 in NIH 3T3 cells (p/n W10-000-358) (lane 3). Peptide competition (lane 4) blocks specific reactivity of the antibody with purified UBC12 protein. Comparison to a molecular weight marker (not shown) indicates a single band of ~21.0 kDa corresponding to the expected molecular weight for the protein. The blot was blocked with 5% non-fat dry milk in TBS supplemented with 0.1% Tween-20 at 4 C overnight. After washes the blot was incubated with a 1:1,000 dilution of the antibody at room temperature for 2 h in TBS-Tween. Washes consisted of 3 changes of TBS-Tween buffer for 15 min each. Detection occurred using HRP anti-Rabbit IgG diluted 1:2,000 and signal processing by chemiluminescence reagent with a 10-sec exposure time. Other detection systems will yield similar results. Personal communication Martine Roussel.
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