This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 575-600 of Saccharomyces cerevisiae (bakers yeast) Mre11 protein.
Konjugation:
Unconjugated
Alternative Synonym:
rabbit anti-Mre11 antibody, Double-strand break repair protein MRE11, DNA recombination and repair protein, Meiotic recombination 11
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Saccharomyces cerevisiae
Antibody Type:
Primary Antibody
Application Verdünnung:
ELISA: 1:10,000 - 1:50,000, ChIP: User Optimized, WB: 1:500- 1:2,000
Anwendungsbeschreibung:
This affinity purified antibody has been tested for use in ELISA and by ChIP assay. Specific conditions for reactivity should be optimized by the end user.
Chromatin Immunoprecipitation (ChIP) using Rocklands Affinity Purified Mre11 (S. cerevisiae) antibody. A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0m lanes) was shifted into galactose containing medium (induced 60m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower band) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR Green (Invitrogen), and detected using a Fuji scanning fluorometer. Personal Communication. Michael Lichten, NIH, CCR, Bethesda, MD.
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